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利用荧光光纤传感器来检测癌症必须弄清楚肝癌细胞与正常肝细胞是否具有不同的荧光特征。在体外培养了SMMC-7721肝癌细胞和HL-7702正常肝细胞,然后用荧光光谱仪对它们的自体荧光进行检测。结果表明,两种细胞的自体荧光发射光谱在550～720 nm范围内发射峰的数目和峰位均相同(602 nm和691 nn),只是主峰的波形略有差异。在720～800 nm范围内,肝癌细胞有一特征荧光峰,峰位置在734 nm附近,而正常肝细胞则无此峰。通过对多代培养的细胞进行荧光光谱测试,发现两种细胞的荧光光谱特征在细胞传代的过程中保持不变,说明细胞中的荧光特征在传代过程中保持稳定。这两方面的实验为利用荧光光纤传感器来检测癌症提供了一定的参考。
The use of fluorescent fiber optic sensors to detect cancer must figure out whether hepatoma cells have different fluorescence characteristics than normal hepatocytes. SMMC-7721 hepatocarcinoma cells and HL-7702 normal hepatocytes were cultured in vitro, and then their autofluorescence was detected by fluorescence spectroscopy. The results showed that the number of emission peak and the peak position of the two kinds of cells in the range of 550-720 nm were the same (602 nm and 691 nn), respectively, except for the main peak. In the range of 720-800 nm, hepatoma cells have a characteristic fluorescence peak with a peak at around 734 nm, whereas normal hepatocytes do not have this peak. Fluorescence spectra of the cultured cells from multiple generations showed that the fluorescence spectra of the two cells remained unchanged during the passage of the cells, indicating that the fluorescence characteristics in the cells remained stable during passage. Both of these experiments provide a reference for the detection of cancer using fluorescence fiber optic sensors.