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目的:探讨黄芪多糖(Astragalus polysaccharide,APS)对异丙肾上腺素(Isoproterenol,ISO)诱导的大鼠心肌肥厚和心肌细胞凋亡的影响。方法:45只SD大鼠随机分为空白对照组、模型对照组、黄芪多糖800mg/kg/d、400 mg/kg/d、200mg/kg/d剂量组。对于模型对照组和给药处理组,腹腔注射异丙肾上腺素10mg/kg/d连续2周制备心肌疾病模型。造模后,对空白对照组和模型对照组给予纯净水,给药处理组分别给予相应浓度的黄芪多糖6周。于第8周末,各组动物分别检测全心质量指数(HMI)和左心质量指数(LVMI);取左心室组织进行HE染色,测量左心室心肌细胞横径(Transverse diameter of left ventricular myocardial cell,TDM);采用TUNEL检测心肌细胞凋亡;Western blot检测bcl-2;bax;caspase-3的蛋白表达。结果:与空白对照组相比,模型对照组大鼠表现为HMI、LVMI、TDM、细胞凋亡率及bax、caspase-3的蛋白表达显著增加,bcl-2的蛋白表达显著降低。与模型对照组相比,黄芪多糖各组表现为HMI、LVMI、TDM、细胞凋亡率及caspase-3的蛋白表达均降低,且黄芪多糖800mg/kg组最显著;bax的蛋白表达都有所降低,且黄芪多糖400mg/kg组最显著;bcl-2蛋白表达都有所升高,且黄芪多糖400mg/kg组最显著。结论:黄芪多糖能改善异丙肾上腺素诱导的心肌肥厚和心肌细胞凋亡,其机制可能是通过影响与细胞凋亡相关的bcl-2、bax和caspase-3的蛋白表达来保护心脏。
Objective: To investigate the effects of astragalus polysaccharide (APS) on cardiac hypertrophy and cardiomyocyte apoptosis induced by isoproterenol (ISO) in rats. Methods: Forty-five SD rats were randomly divided into blank control group, model control group, Astragalus polysaccharide 800mg / kg / d, 400 mg / kg / d, 200mg / kg / d. For the model control group and the administration group, a myocardial disease model was prepared by intraperitoneally injecting isoproterenol 10 mg / kg / d for 2 weeks. After modeling, the blank control group and model control group were given pure water, the treatment group were given the corresponding concentration of astragalus polysaccharide for 6 weeks. At the end of the 8th week, the whole heart mass index (HMI) and left ventricular mass index (LVMI) of the animals in each group were measured. Left ventricular tissue was taken for HE staining to measure the diameter of left ventricular myocardial cells TDM). Cardiomyocyte apoptosis was detected by TUNEL. The protein expression of bcl-2, bax and caspase-3 were detected by Western blot. Results: Compared with the blank control group, the expression of caspase-3, HMI, LVMI, TDM, apoptosis rate and the expression of bax were significantly increased in the model control group, while the protein expression of bcl-2 was significantly decreased. Compared with the model control group, the APS, HMI, LVMI, TDM, apoptosis rate and the expression of caspase-3 protein in Astragalus polysaccharide group were all lower than those in the model control group, and Astragalus polysaccharide 800mg / kg group was the most significant; Decreased, and Astragalus polysaccharides 400mg / kg group the most significant; bcl-2 protein expression increased, and astragalus polysaccharide 400mg / kg group the most significant. Conclusion: Astragalus polysaccharide can improve isoproterenol-induced cardiac hypertrophy and cardiomyocyte apoptosis possibly through protecting the heart by affecting the protein expression of bcl-2, bax and caspase-3 related to apoptosis.