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目的 :研究抗独特型抗体佐剂疫苗 (Ab2 +SAF)对膀胱癌的免疫治疗作用 ,为临床实验提供动物实验依据。方法 :用抗人膀胱癌Ab2 +SAF免疫BALB/C小鼠为实验组 ;对照组用生理盐水。免疫 3次后 ,于末次免疫后 1周 ,将新鲜人膀胱移行细胞癌组织移植于小鼠肾包膜下 ,分别于移植后第 2、4、6、8和 10天处死小鼠 ,取血清进行抗抗独特型抗体 (Ab3 )分析。移植瘤行组织学检查 ,观察宿主淋巴细胞浸润情况及瘤细胞可见率。结果 :实验组小鼠肾包膜下人膀胱癌细胞很快受到排斥 ,在移植后第 6天 ,淋巴细胞浸润即达高峰 ,而对照组在第10天才达高峰 ;瘤细胞可见率 ,在移植后第 6天即明显降低 ,而对照组呈逐渐下降。实验组Ab3 为阳性 ,而对照组为假阳性或阴性。结论 :Ab2 作为抗原免疫小鼠后 ,能够排斥瘤细胞的生长。
Objective: To study the immunotherapy effect of anti-idiotypic antibody adjuvant vaccine (Ab2 + SAF) on bladder cancer and to provide animal experimental evidence for clinical trials. Methods: BALB / C mice were immunized with anti-human bladder cancer Ab2 + SAF as experimental group and control group with saline. After three times of immunization, fresh human bladder transitional cell carcinoma tissue was transplanted into the mouse renal capsule 1 week after the last immunization, mice were sacrificed on the 2nd, 4th, 6th, 8th, and 10th day after transplantation, respectively. The serum Anti-idiotypic antibody (Ab3) analysis was performed. The xenografts were histologically examined to observe the host lymphocyte infiltration and the tumor cell visible rate. Results: The subcutaneous renal bladder cancer cells in the experimental group were quickly rejected. On the 6th day after transplantation, the lymphocyte infiltration reached the peak, while the control group reached the peak on the 10th day. The tumor cell visible rate, After 6 days was significantly reduced, while the control group showed a gradual decline. The experimental group Ab3 was positive, while the control group was false positive or negative. Conclusion: Ab2 can immunize mice against the growth of tumor cells.