目的:探讨核因子-κB(NF-κB)和血管内皮生长因子(VEGF)在非小细胞肺癌中的表达及其与肿瘤血管形成的关系。方法:应用免疫组织化学方法检测56例非小细胞肺癌及20例癌旁肺组织中的NF-κB P65、VEGF的表达,并用抗CD34测定肿瘤血管的密度(MVD)。结果:(1)在非小细胞肺癌中NF-κB P65、VEGF的表达阳性率分别为83.9%(47/56)、69.6%(39/56),明显高于癌旁组织(P<0.05);(2)NF-κB P65的表达在不同的TNM分期、淋巴结及胸腔积液、吸烟的分组之间差异有统计学意义,VEGF的表达在淋巴结及胸膜转移之间差异有统计学意义;(3)NF-κB P65、VEGF、MVD三者间存在明显相关性。结论:NF-κB、VEGF异常表达与NSCLC的发生、发展及肿瘤血管的形成密切的关系。 Objective: To investigate the expression of nuclear factor-κB (NF-κB) and vascular endothelial growth factor (VEGF) in non-small cell lung cancer (NSCLC) and its relationship with tumor angiogenesis. Methods: The expressions of NF-κB P65 and VEGF in 56 cases of non-small cell lung cancer and 20 cases of paracancerous lung tissues were detected by immunohistochemistry. The density of tumor vessels (MVD) was measured by anti-CD34. Results: (1) The positive rates of NF-κB P65 and VEGF in non-small cell lung cancer were 83.9% (47/56) and 69.6% (39/56), respectively, which were significantly higher than those in paracancerous tissues (P <0.05) ; (2) The expression of NF-κB P65 in different TNM stage, lymph node and pleural effusion, smoking group differences was statistically significant, VEGF expression in lymph node and pleural metastasis was statistically significant; ( 3) NF-κB P65, VEGF, MVD there is a clear correlation between the three. Conclusion: The abnormal expressions of NF-κB and VEGF are closely related to the occurrence and development of NSCLC and the formation of tumor vessels.