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应用ERIC-PCR指纹技术,对采自四川省6个籼稻自然生态区的60个稻曲病菌菌株进行了DNA分子水平上的遗传多样性研究,并进行UPGMA聚类分析和相似性分析。结果表明,所采用的ERIC引物在不同供试菌株中分别扩增出5～20条不等的带谱;在0.75相似水平上,供试菌株被划分为11个遗传型群,其中L1、L4、L5为优势群,并存在次要小型群和特异性型群;来自同一地区的稻曲病菌菌株具有较高的遗传相似性,而不同地区的稻曲病菌则表现出程度不同的变异,且寄主品种与稻曲病菌遗传差异之间的相关性较小。
The ERIC-PCR fingerprinting was used to study the genetic diversity of 60 strains of Y. tomentosa isolates collected from 6 indica rice natural ecological regions in Sichuan Province, and UPGMA cluster analysis and similarity analysis were performed. The results showed that ERIC primers were used to amplify 5 to 20 bands in different strains. At the similar level of 0.75, the tested strains were divided into 11 genetic groups, in which L1, L4 , L5 was the dominant group, and there were minor small groups and specific groups; the strains of Y. tomentosa isolated from the same area had higher genetic similarity, while the strains from different regions showed different degrees of variation, and The correlation between the host varieties and the genetic difference of V. smigata was minor.