作者选用MRC-5细胞及水痘-带状疱疹病毒(VZV)Hallworth株作为制备疫苗的细胞系和病毒株,通过去污剂和甲醛处理,蔗糖超速离心和丙酮沉淀制备出每剂分别含210、240、250和280μg蛋白(相当于1×10~7VZV感染细胞)的四批疫苗.该法保留了VZV蛋白和一些特异性糖蛋白的免疫原性,并使病毒DNA小于20pg/250μg蛋白(假定为1剂 The authors selected MRC-5 cells and the Hallworth strain of varicella-zoster virus (VZV) as the vaccine-producing cell lines and virus strains. Each of the two formulations was prepared by detergent and formaldehyde treatment, ultracentrifugation with sucrose, and acetone precipitation. Four batches of vaccine containing 240, 250 and 280 μg of protein (equivalent to 1 × 10 -7 VZV infected cells), which retains the immunogenicity of VZV proteins and some specific glycoproteins and makes the viral DNA less than 20 pg / 250 μg protein For 1 agent