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c AMP反应成分结合蛋白 (c AMP response elem ent-binding protein,CREB)是一种转录因子 ,它在磷酸化之后可调节靶基因的转录。 CREB在 13 3位置的丝氨酸的磷酸化 ,与脊髓中伤害性传入的处理有关 ,本文作者等用特殊抗体对此进行了免疫细胞化学研究。在正常大鼠 ,虽然几乎所有脊髓神经元的核中都可见 CREB的轻度着色 ,但磷酸化的 CREB仅见于双侧腰段脊髓的 ～ 层 (75± 15 vs60± 18)和 层 (9± 3 )。用福尔马林注射引起一侧后脚掌出现炎症时 ,可在双侧腰段脊髓看到磷酸化CREB细胞核的快速 (小于 5 min)和节段性的显著增多 ;它们主要分布在双侧背角表层 ～ 层 (2 5 4± 2 0 vs 2 62± 2 3 )、 层(115± 13 )和双侧 ～ 层 (3 46± 2 0 vs3 2 8± 2 6) ;而在对照和炎症组大鼠的胸段脊髓中均未见磷酸化 CREB的增加。在注射CFA诱发一侧炎症或切断一侧坐骨神经的实验组大鼠 ,也可看到至少延续到第三天的强而双侧性的 CREB的磷酸化。这种由一侧后肢伤害性传入引致腰段脊髓中镜像式双侧 CREB磷酸化的出现 ,与一般看到的损伤传入只在同侧脊髓背角引起某些神经化学改变的结果不同 ,可能是人神经损伤后或在实验动物中出现对侧镜像式疼痛过敏现象的基础
c AMP response eleme-binding protein (CREB) is a transcription factor that regulates the transcription of a target gene after phosphorylation. The serine phosphorylation of CREB at the 13 3 position is related to the treatment of nociceptive afferent in the spinal cord, and the authors performed immunocytochemistry studies with specific antibodies. In normal rats, although mild staining of CREB was observed in almost all of the nuclei of spinal cord neurons, phosphorylated CREB was found only in the layers (75 ± 15 vs 60 ± 18) and layers (9 ± 15) of the bilateral lumbar spinal cord 3). When formalin injection causes inflammation in one of the hind paws, a rapid (less than 5 min) and segmental increase in the phosphorylated CREB nuclei can be seen in the bilateral lumbar spinal cord; they are mainly distributed in the bilateral dorsal (2 54 ± 20 vs 22 62 ± 23), layer (115 ± 13) and bilateral layers (466 ± 20 vs 32 8 ± 26) in the control group and the inflammation group No increase in phosphorylated CREB was found in the thoracic spinal cord of rats. In the experimental group injected with CFA-induced inflammation on the side or sciatic nerve in the unilateral sciatic nerve, the strong and bilateral phosphorylation of CREB was observed at least until the third day. The appearance of double-sided CREB phosphorylation in the lumbar spinal cord resulting from the nociceptive afferent of one hind limb differs from the generally seen impairment of afferent afferent afferent changes that cause some neurochemical changes only in the ipsilateral spinal dorsal horn, May be the basis of the contralateral mirror-type hyperalgesia following injury to the human nerve or in experimental animals