关于碳氧血红蛋白(HbCO)测定采用的方法有定性法,分光光度法和加温定量法,虽然方法较多,但至今尚未有一个较理想的简便方法,对分光光度法来说,虽方法简便,用血量少,且可取末稍血,但还存在着标准曲线钭率太小,不灵敏且重现性不够理想,不易控制好,此外用加温定量法,需用正常人血作对照,用血量过大,这就给实验带来了不便。为了寻找一个较为理想的方法,我们对加温定量法作了改进,在测定时减少了用血量,利用自身血液作为对照,且可直接计算结果。经实验证明,此法操作简便,不需特殊仪器,又可以较准确的定量测出血中HbCO浓度,供 About the determination of carboxyhemoglobin (HbCO) using the methods of qualitative, spectrophotometric and quantitative heating method, although the method is more, but so far there is no more an ideal simple method for spectrophotometry, although the method is simple , With less blood, and desirable for the end of the blood, but there is still a standard curve 钭 rate is too small, insensitive and reproducible less than ideal, not easy to control, in addition to using warm quantitative method, the need for normal blood control , With excessive blood, which brought inconvenience to the experiment. In order to find a more ideal method, we improved the method of warming and quantifying, reducing the amount of blood used in the measurement and using our own blood as a control, and we can directly calculate the result. The experiment proved that this method is simple, without special equipment, but also a more accurate quantitative measurement of blood HbCO concentration for