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建立了毛细管电泳法快速测定猪体组织中甜菜碱含量的方法。甜菜碱首先转化为苯甲酰甲基酯后直接上样测定。pH为 3 .0的磷酸缓冲溶液使甜菜碱酯化物和甜菜碱结构类似物酯化物之间以及酯化物和酯化剂之间都能很好地分离 ,这也省去了反应混合液的前处理。该法标准曲线的线性范围为 4～ 6 0 0mg L ,相关系数r为 0 .9999,最低检测限为 1mg L ,相对标准偏差为 2 .2 %～ 4.7% ,标准加入回收率为 95 9%～ 98.4%
A rapid method for the determination of betaine in pig tissues by capillary electrophoresis was established. Betaine is first converted to phenacyl ester directly after the determination of the sample. A phosphate buffer solution at a pH of 3.0 provided a good separation between the betaine ester and the betaine analogs and between the esterification and the esterification agent, deal with. The calibration curve of this method has a linear range of 4 ~ 600 mg L, a correlation coefficient of 0.9999, a minimum detection limit of 1 mg L, a relative standard deviation of 2.2% -4.7% and a standard recovery of 95.9% ~ 98.4%