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目的研究慢性低氧时大鼠血浆腺苷水平的变化及其机制。方法将42只雄性SD大鼠随机分成常氧组(对照组),低氧1 d、3 d、5 d、7 d、14 d、21 d组(H1、H3、H5、H7、H14、H21组),每组6只。分别于低氧第1天、第3天、第5天、第7天、第14天及第21天测定各组大鼠血浆腺苷水平、腺苷脱氨酶(ADA)活性以及血浆中5’-核苷酸酶(5’-NT)水平。应用SPSS 16.0软件进行统计学分析。结果低氧1 d后大鼠血浆腺苷水平开始升高,于第3天达到最高峰,与对照组比较差异有统计学意义(P<0.001);此后,随着低氧时间的延长,血浆腺苷水平逐渐下降,第14天及第21天与对照组比较差异均无统计学意义(Pa>0.05)。血浆5’-NT水平在低氧第3天增高最显著(P<0.001),在低氧3 d后逐渐下降,但仍高于对照组(P<0.001)。血浆ADA活性在慢性低氧第1天、第3天较对照组明显增高(Pa<0.001),随着低氧时间的延长,其活性稍下降,但仍明显高于对照组(P<0.001)。结论慢性低氧在早期可引起血浆腺苷水平增加,后期血浆腺苷水平逐渐降低,其变化与5’-NT水平和ADA活性有关。
Objective To study the changes of plasma adenosine level in rats with chronic hypoxia and its mechanism. Methods Forty-two male Sprague-Dawley rats were randomly divided into normoxia group (control group), hypoxia group on day 1, day 3, day 5, day 7, day 14 and day 21 (H1, H3, H5, H7, H14, H21 Group), 6 in each group. The levels of adenosine deaminase (ADA) and the activities of adenosine deaminase (ADA) in plasma of rats in each group were measured on hypoxia day 1, day 3, day 5, day 7, day 14 and day 21 ’- nucleotidase (5’-NT) levels. Application SPSS 16.0 software for statistical analysis. Results After 1 day of hypoxia, the plasma adenosine level began to rise and peaked on the 3rd day, which was significantly different from that of the control group (P <0.001). After that, with the prolongation of hypoxia time, plasma The level of adenosine gradually decreased. There was no significant difference between the 14th and the 21st day and the control group (P> 0.05). The level of 5’-NT in plasma increased most significantly (P <0.001) on the 3rd day of hypoxia and gradually decreased after 3 days of hypoxia, but still higher than that of the control group (P <0.001). Plasma ADA activity was significantly higher (P <0.001) on day 1 and day 3 of chronic hypoxia than that of control group (P <0.001), and decreased slightly with the prolongation of hypoxia time, but still significantly higher than that of control group (P <0.001) . Conclusions Chronic hypoxia can cause the increase of plasma adenosine level in early stage and the gradual decrease of plasma adenosine level in late stage, which is related to 5’-NT level and ADA activity.