Tetracycline selective electrode using molecularly imprinted polymer particles as quasi-ionophore was constructed the first time,and its performance was carefully characterized.Due to the specific recognition of tetracycline by the particles,the selectivity coefficients for routine interferences were less than 10-4.Benefited from the absence of tetracycline in the sensitive membrane and the optimized composition of the inner filling solution,the limit of detection of the electrode was reduced to about 2.5 × 10-8 mol/L.It exhibited a good electrode slope 57.6 mV/decade near the theoretical Nernstian one,with a wide linear working range from6.0 × 10-8 to 1.0 × 10-3 mol/L.The fabricated electrode should be used in pH 2-4,response time of which was less than 200 s when the concentration of tetracycline was higher than 1.0 × 10-6 mol/L and no more than 30 min at the concentration of1.0 × 10-8 mol/L.