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目的:观察α-突触核蛋白(α-Synuclein,α-Syn)对小鼠空间学习记忆能力的影响。方法:以C57/B6野生型小鼠(Wild Type,WT)及α-Syn过表达转基因小鼠(Tg-α-Syn)为研究对象,采用Morris水迷宫(Morris water maze,MWM)中定位航行实验、空间探索实验以及可视平台实验,按照训练要求,记录两组小鼠游泳路径和完成任务的时间。结果:在定位航行试验中,随着训练时间的增加,两组小鼠的寻台潜伏期均缩短。同时,在训练第五天时,与WT组小鼠相比,Tg-α-Syn小鼠的寻台潜伏期明显延长(58.77±1.32 versus 17.34±6.44,*P<0.05)。在空间探索实验中,与WT组小鼠相比,Tg-α-Syn小鼠在目标象限停留时间更短(17.91±2.14versus 5.59±4.98,*P<0.05)。为了进一步证明Tg-α-Syn小鼠的空间记忆与学习能力下降是与海马相关,采用了可视平台实验,结果显示:两组小鼠的寻台潜伏期无显著性差异(P>0.05),即Tg-α-Syn小鼠不存在运动障碍和视觉障碍。结论:α-Syn过表达可导致海马相关的空间记忆、学习能力受损。
Objective: To observe the effect of α-synuclein (α-Syn) on spatial learning and memory in mice. Methods: The C57 / B6 wild type (WT) and α-Syn overexpression transgenic mice (Tg-α-Syn) were used as research objects, and the animals were maneuvered by Morris water maze (MWM) Experiments, space exploration experiments and visual platform experiments, according to training requirements, record two groups of mice swimming path and time to complete the task. Results: In the positioning navigation test, the latency of the two groups of mice was shortened as the training time increased. Meanwhile, on the fifth day of training, Tg-α-Syn mice showed significantly longer latency (58.77 ± 1.32 versus 17.34 ± 6.44, * P <0.05) compared with WT mice. In space exploration experiments, Tg-α-Syn mice had shorter residence times in the target quadrant compared with WT mice (17.91 ± 2.14 vs. 5.59 ± 4.98, * P <0.05). In order to further prove that the decline of spatial memory and learning ability of Tg-α-Syn mice is related to the hippocampus, a visual platform experiment was used. The results showed that there was no significant difference in search latency between the two groups (P> 0.05) That is, Tg-α-Syn mice do not have dyskinesias and visual disturbances. Conclusion: Overexpression of α-Syn can lead to hippocampal-related spatial memory and impaired learning ability.