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通过ELIB技术对曼氏裂头蚴可溶性抗原的免疫学特性进行分析,并用ELISA法对特异性抗体作了检测。结果表明:裂头蚴抗原内有5种组分被感染兔血清识别,其中低分子量组分是主要的血清学抗原;裂头蚴感染可使宿主产生高水平的抗体反应;用ELISA法诊断裂头蚴感染简便可行。
The immunological characteristics of soluble antigen of Manfin spider larvae were analyzed by ELIB and the specific antibodies were detected by ELISA. The results showed that there were five components in the antigen of the sporozoite, which were identified by the sera of infected rabbits. Among them, the low molecular weight fraction was the major serological antigen. The infection of the fission larvae could make the host produce high level of antibody reaction. The diagnosis of the infection by the ELISA was simple and feasible.