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目的探究何首乌饮影响大鼠睾丸间质(Leydig)细胞类固醇激素合成急性调节蛋白(St AR)和细胞色素P450胆固醇侧链裂解酶(P450scc)蛋白表达的变化。方法选用10~20 d的幼年雄性Wistar大鼠,分离、培养Leydig细胞,然后收集。采用氧化损伤的方法建立Leydig细胞衰老模型。实验分组:间质细胞衰老模型组,何首乌饮处理组,首乌丸处理组,正常组和何首乌饮对照组。结果衰老模型组Leydig细胞St AR和P450scc的免疫组织化学染色强度明显低于正常组和何首乌饮对照组(P<0.01)。另一方面,何首乌饮和何首乌丸干预组St AR和P450scc的免疫组织化学染色强度明显高于衰老模型组(P<0.05),何首乌饮组好于何首乌丸组。结论何首乌饮可提高睾丸Leydig细胞St AR和P450scc蛋白的表达,提高睾酮的合成能力,从而延缓Leydig细胞衰老。
Objective To investigate the changes of the protein expression of StAR and P450scc in Leydig cells of rats induced by Polygonum multiflorum Thunb. Methods Juvenile male Wistar rats aged 10-20 days were used to isolate and culture Leydig cells and collect them. Acute Leydig cell senescence model was established by oxidative damage. Experimental group: interstitial cell aging model group, Polygonum drink treatment group, Shouyao Wan treatment group, the control group and the control group. Results The intensity of immunohistochemical staining of St AR and P450scc in Leydig cells of aging model group was significantly lower than that of normal group and control group (P <0.01). On the other hand, the intensity of immunohistochemical staining of St AR and P450scc was significantly higher in the HL group (P <0.05), and the HL group was better than that in the HL group. Conclusion Polygonum multiflorum Thunb can increase the expression of St AR and P450scc protein in testicular Leydig cells, increase the testosterone synthesis ability and delay the aging of Leydig cells.