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目的探讨在促癌剂协同下Ｅｐｓｔｅｉｎ－Ｂａｒ病毒（ＥＢＶ）对人胚鼻咽上皮的感染和转化能力。方法体外培养的人胚鼻咽上皮分别接受ＥＢＶ、ＥＢＶ＋佛波酯（ＴＰＡ）或ＴＰＡ处理，不同时间测量细胞生长晕大小，收集细胞，涂片，ＨＥ染色作形态测量；移植了人胚鼻咽组织的裸小鼠分别接受多次ＥＢＶ、ＥＢＶ＋ＴＰＡ皮下注射，处死动物后对移植物作常规病理切片观察。结果体外培养的人胚鼻咽上皮在ＥＢＶ转染后（单用ＥＢＶ或在ＴＰＡ协同下），生长能力增加，核浆比增大；在体内，ＥＢＶ感染（单用ＥＢＶ或在ＴＰＡ协同下）可引起移植于裸小鼠的人胚鼻咽组织癌变（原位癌与早期浸润癌各１例）。在体外，单独使用ＴＰＡ没有诱癌作用，但似能增加ＥＢＶ的感染率，尤其在早期；在体内，在ＴＰＡ协同下，ＥＢＶ能诱发早期浸润癌。结论提示ＥＢＶ在体外能使人胚鼻咽上皮细胞出现恶性转化倾向，及引起移植于裸小鼠的人胚鼻咽组织癌变，ＴＰＡ能促进ＥＢＶ感染和致癌作用。
Objective To investigate the ability of Epstein-Bar virus (EBV) to infect and transform human embryonic nasopharyngeal epithelium under the cooperation of cancer promoters. Methods Human embryonic nasopharyngeal epithelial cells cultured in vitro were treated with EBV, EBV + phorbol ester (TPA) or TPA respectively. The size of cell growth halo was measured at different times, and the cells, smears and HE staining were collected for morphology measurement. Tissues of nude mice received multiple subcutaneous injections of EBV and EBV + TPA, respectively. The animals were sacrificed and the grafts were examined by routine histopathology. Results In vitro, EBV (EBV alone or in the presence of TPA) EBV-infected human EBV-derived human nasopharyngeal epithelial cells grew in vitro with enhanced EBV or TPA cytotoxicity. Can cause transplanted human embryo nasopharyngeal carcinoma in nude mice (in situ carcinoma and early invasive carcinoma in 1 case). In vitro, TPA alone does not induce cancer but appears to increase EBV infection, especially in the early stages; EBV induces early invasive carcinoma in vivo in the presence of TPA in vivo. The results suggest that EBV can induce malignant transformation of human nasopharyngeal epithelial cells in vitro and cause carcinogenesis of human embryonic nasopharyngeal tissues transplanted in nude mice. TPA can promote EBV infection and carcinogenesis.