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以北沙参叶片和茎段为外植体,采用MS和B5培养基对其进行愈伤组织诱导,继代培养后进行悬浮培养,研究了不同浓度碳源、6-BA和NAA对其细胞生长的影响,以期为进一步建立北沙参细胞培养体系以及提高其次生代谢物产量奠定试验基础。结果表明:1/2MS+0.4mg/L 6-BA+1.5mg/L NAA琼脂培养基为北沙参愈伤组织诱导的较适宜培养基;1/2MS+0.2mg/L 6-BA+1.2mg/L NAA为适宜的继代培养基;在细胞悬浮培养过程中,分别添加0.2mg/L 6-BA、1.6mg/L NAA和20g/L蔗糖有利于细胞的生长。
The leaves and stem segments of Radix Aconiti Kusnezoffii as explants were induced by MS and B5 medium, subcultured and then cultured in suspension. The effect of different concentrations of carbon source, 6-BA and NAA on the growth of their cells In order to lay the foundation for the further establishment of cell culture system and the increase of its secondary metabolites. The results showed that 1 / 2MS + 0.4mg / L 6-BA + 1.5mg / L NAA agar medium was the suitable culture medium for callus induction of NAFG; 1/2 MS + 0.2mg / L 6-BA + 1.2 mg / L NAA was the suitable subculture medium. During the process of cell suspension culture, adding 0.2mg / L 6-BA, 1.6mg / L NAA and 20g / L sucrose, respectively, was conducive to cell growth.