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目的研究血吸虫感染BALB/c鼠后,虫卵在其部分组织中特别是在肠壁中的分布状况。方法 10只BALB/c鼠腹部贴片感染血吸虫尾蚴(40±1条/只),感染42d后剖杀,分别取鼠的肝脏、脾脏、肠道(小肠上段、中段、下段、盲结肠、直肠)进行成虫计数、虫卵计数、毛蚴孵化、组织切片观察,并将得到的结果进行统计学分析。结果血吸虫虫卵在BALB/c鼠各组织的分布为:肝脏(59.64%)、脾脏(0.81%)、小肠上段(6.27%)、小肠中段(5.24%)、小肠下段(5.14%)、盲结肠(17.48%)、直肠(5.41%),肝脏的沉积虫卵数和孵化数与其他组织相比差异显著,肠组织中大肠段比小肠段的沉积虫卵数和孵化数多且差异显著。在孵化率的比较中肝脏的孵化率最高(1.09%)。病理切片观察,肝脏产生大量肉芽肿结节,肠壁出现不同程度的炎症坏死。结论 BALB/c鼠定量人工感染血吸虫后,肝脏中的沉积虫卵最多,其次是盲结肠,最少的是脾脏;且不同组织的沉积虫卵数目与病理损伤程度有关。
Objective To study the distribution of the eggs in some tissues of the BALB / c mice infected with Schistosoma japonicum, especially in the intestinal wall. Methods Totally 40 BALB / c mice were infected with cercariae (40 ± 1) by abdominal patch and were sacrificed after 42 days. The liver, spleen, intestine (upper intestine, middle segment, lower segment, ) For adult counting, egg count, miracidia hatching, histological observation, and the results obtained for statistical analysis. Results The distribution of Schistosoma japonicum eggs in BALB / c mice was as follows: liver (59.64%), spleen (0.81%), upper intestine (6.27%), middle intestine (5.24%), lower intestine (17.48%), rectum (5.41%), and the number of eggs and hatching in the liver were significantly different from those in other tissues. The numbers of eggs and hatching in the large intestine of intestine were significantly higher than those in small intestine. The hatchability of the liver was the highest in the hatchery rate (1.09%). Pathological observation, the liver produces a large number of granuloma nodules, varying degrees of inflammation and necrosis of the intestinal wall. Conclusions After the artificial infection of Schistosoma japonicum by BALB / c mice, the number of deposited eggs in the liver is the largest, followed by the blind colon, and the spleen is the least. The number of deposited eggs in different tissues is related to the degree of pathological injury.