结合近几年的高考试题可发现对基因工程考查包括:DNA重组技术的基本工具及其使用,目的基因的获取与扩增,基因表达载体的构建,基因进入受体细胞的载体与途径,目的基因表达与检测、蛋白质工程的原理等。其中涉及限制性核酸内切酶相关的试题最多。结合相关试题总结如下。一、限制性核酸内切酶的来源和特点及与其他酶的区别限制性核酸内切酶是从原核生物中分离 In combination with the entrance examination papers in recent years, we can find that the genetic engineering examination includes the basic tools of DNA recombination technology and its use, the acquisition and amplification of the target gene, the construction of the gene expression vector, the vector and the pathway of the gene into the recipient cell, Gene expression and detection, the principle of protein engineering and so on. Which involves restriction endonuclease related questions most. The related questions are summarized as follows. A source of restriction endonucleases and characteristics and differences with other enzymes Restriction endonucleases are isolated from prokaryotes