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目的探讨Apr-1基因调控QBC939胆管癌细胞周期的机制。方法运用脂质体介导法将Apr-1基因转染胆管癌细胞系QBC939,建立稳定表达Apr-1基因的细胞模型(QBC939-Apr-1)。采用细胞周期基因芯片,观察转染Apr-1基因前后胆管癌细胞细胞周期相关基因表达的变化。结果转染Apr-1基因的QBC939-Apr-1细胞出现Apr-1 mRNA的表达,成功建立了稳定表达Apr-1基因的细胞模型。基因芯片检测结果发现:Skp2及UBE基因的表达水平明显上调,而CDC6,Cyclin H等25种基因的表达下降,其中MRE11A,CKS2,CDK8及CDC45等下调在3倍以上。结论过表达的Apr-1基因可诱导QBC939细胞多种细胞周期相关基因表达发生变化,为深入认识Apr-1基因参与调节细胞周期的机制提供了新的思路。
Objective To investigate the mechanism of Apr-1 gene regulating the cell cycle of QBC939 cholangiocarcinoma. Methods Apr-1 gene was transfected into cholangiocarcinoma cell line QBC939 by liposome-mediated method to establish a cell model stably expressing the gene Apr-1 (QBC939-Apr-1). Cell cycle gene chips were used to observe the changes of cell cycle related gene expression in the cholangiocarcinoma cells transfected with Apr-1 gene. Results The expression of Apr-1 mRNA was detected in QBC939-Apr-1 cells transfected with Apr-1 gene. The cell model stably expressing Apr-1 gene was established successfully. The results of gene microarray showed that the expressions of Skp2 and UBE genes were significantly up-regulated while the expressions of 25 genes such as CDC6 and Cyclin H were down-regulated. The down-regulation of MRE11A, CKS2, CDK8 and CDC45 was over 3 times. Conclusion The overexpression of Apr-1 gene can induce the changes of many cell cycle-related genes in QBC939 cells, which provides a new idea for understanding the mechanism of Apr-1 gene involved in the regulation of cell cycle.