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目的为了解河南省肉鸡养殖和屠宰加工过程中弯曲菌污染状况,为食品安全风险评估提供数据支持。方法选择肉鸡存栏量和屠宰量占全省60%以上的养殖场和屠宰场,在养殖环节采集鸡肛拭子,屠宰环节采集预冷后分割前鸡胴体,按照GB/T 4789.9-2008进行定性检测,按照ISO/TS 10272-3进行定量检测。对分离菌株用PCR方法检测HIP、CC和CL基因进行弯曲菌的复核。结果鸡肛拭子弯曲菌检出率为31.1%,胴体检出率为38.7%,仅检出空肠和结肠弯曲菌。鸡胴体弯曲菌污染严重,数量大于500 MPN/g的占75.7%。肛拭子和胴体的检出率随月份出现相似的波动。所有检出空肠弯曲菌均具有HIP基因,所有结肠弯曲菌均具有CC基因。结论弯曲菌在鸡胴体污染严重,活鸡携带弯曲菌是胴体污染的源头,屠宰过程存在严重的环境和交叉污染,建议监督部门加大监管力度,企业增强自律和食品安全意识,把食品安全隐患消除在初始阶段。
Objective To understand the status of Campylobacter bacteria contamination in broiler breeding and slaughter and processing in Henan Province and provide data support for food safety risk assessment. Methods The farms and slaughterhouses which accounted for more than 60% of the total stock in the province were selected for stocking and slaughtering of broiler chickens. The anal swabs were collected during the breeding period. The pre-chilled carcasses were collected after slaughtering and were sorted according to GB / T 4789.9-2008 Testing, quantitative testing according to ISO / TS 10272-3. Strains of Campylobacter were screened by PCR for HIP, CC and CL genes. Results The detection rate of Campylobacter jejuni was 31.1%, and the detection rate of carcass was 38.7%. Only Campylobacter jejuni and Campylobacter coli were detected. Chicken carcases Campylobacter contamination is serious, the number of more than 500 MPN / g accounted for 75.7%. The detection rates of anal swabs and carcasses fluctuate similarly with the month. All Campylobacter jejuni strains have HIP gene, and all C. Coli have CC gene. CONCLUSION: Campylobacter is seriously polluted in chicken carcasses. Campylobacter livelihood is the source of carcasses. There are serious environmental and cross-contamination in slaughter process. It is suggested that supervision departments should step up supervision and control, and enterprises should strengthen self-discipline and food safety awareness so that food safety risks Elimination in the initial stage.