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目的 探讨婴幼儿轮状病毒无症状感染的状况和意义。方法 采用聚丙烯酰胺凝胶电泳 (PAGE)技术 ,对 5 0 9例非腹泻婴幼儿粪便标本进行检测 ;同时用 10 0例经酶联免疫吸附试验测定(ELISA)轮状病毒阳性的婴幼儿轮状病毒肠炎的腹泻粪便标本作电泳型对照。结果 婴幼儿轮状病毒无症状感染率为 18.47% (94/ 5 0 9)。流行期为 2 3 .2 5 % (93/ 40 0 )远高于非流行期的 0 .92 % (1/10 9) ,P <0 .0 0 1;流行早期为 30 .83% (4 1/ 133)明显高于流行末期的 16 .70 % (2 2 / 131) ,P <0 .0 5 ;不同流行年份婴幼儿轮状病毒无症状感染率差异有非常显著性 (P <0 .0 0 1)。婴幼儿轮状病毒无症状感染的RNA电泳型的主要构成与同期轮状病毒有症状感染的RNA电泳型比较差异无显著性 (P >0 .0 5 )。结论 流行早期婴幼儿轮状病毒无症状感染率的高低和电泳型的主要构成可预测当年轮状病毒感染流行的规模和电泳型
Objective To investigate the status and significance of asymptomatic infection of infants with rotavirus. Methods A total of 509 cases of non-diarrhea infants and young children were examined by polyacrylamide gel electrophoresis (PAGE). In addition, 100 cases of rotavirus-positive infants and young children were tested by enzyme-linked immunosorbent assay (ELISA) Virulent enteritis diarrhea stool specimens for electrophoresis control. Results Asymptomatic infection rate of infants with rotavirus was 18.47% (94/509). The epidemic period was 23.5% (93/40 0) which was much higher than the non-epidemic period 0.92% (1/10 9), P <0.01; the prevalence was 30.83% (4 1/133) was significantly higher than the epidemic end of the 16.70% (2 2/131), P <0. 05; different prevalence of infant rotavirus asymptomatic infection rate differences were significant (P <0. 0 0 1). The main components of RNA electrophoresis of asymptomatic infection of infants and young children were not significantly different from the RNA electrophoresis of symptomatic rotavirus infection over the same period (P> 0.05). Conclusion The prevalence of asymptomatic infection of rotavirus in infants and young children at the early stage of epidemic and the main composition of electrophoresis type can predict the prevalence of rotavirus infection and the type of electrophoresis