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[Objective]This study aimed to investigate the effects of carbon ion implantation and implantation times on growth and genetic variation of sunflowers. [Method]Carbon ions were implanted into Bakui 138,Bakui 136 and Bakui 118 seeds at dose of 5×1013 C/cm2,before they were planted. Their F1-generation seeds were irradiated again. Seeds of the both generations were planted and the growth of the seedlings was observed in field tests. Finally,their genetic variation was analyzed through RAPD. [Result] The germination rate and several agronomic traits like plant height,stem diameter,leaf number and yields of Bakui 138 of once-irradiated group were significantly improved,while that of twice-irradiated group showed opposite trend. The variation of Bakui 136 and Bakui 118 was insignificant. At the molecular level,the genetic distance with the control group of once and twice-irradiated groups was 0. 111 1,0. 108 7 in Bakui 138; 0. 068 0, 0. 030 3 in Bakui 136 and 0. 062 5,0. 043 5 in Bakui 118. [Conclusion]Carbon ion implantation had a significant effect on the growth and development of Bakui 138,and the effect varied with irradiation times. Moreover,it caused genomic variation in the three sunflower cultivars.
[Objective] This study aimed to investigate the effects of carbon ion implantation and implantation times on growth and genetic variation of sunflowers. [Method] Carbon ions were implanted into Bakui 138, Bakui 136 and Bakui 118 seeds at dose of 5 × 1013 C / cm2, before they were planted. Their F1-generation seeds were irradiated again. Seeds of the second generation were planted and the growth of the seedlings was observed in field tests. Finally, their genetic variation was washed through RAPD. [Result] The germination rate and several agronomic traits like plant height, stem diameter, leaf number and yields of Bakui 138 of once-irradiated group were significantly improved, while that of twice-irradiated group showed opposite trend. The variation of Bakui 136 and Bakui 118 was insignificant. At the molecular level, the genetic distance with the control group of once and twice-irradiated groups was 0.111 1,0. 108 7 in Bakui 138; 0. 068 0, 0.0303 in Bakui 136 and 0. 062 5 , 043 5 in Bakui 118 . [Conclusion] Carbon ion implantation had a significant effect on the growth and development of Bakui 138, and the effect varied with irradiation times. Moreover, it caused genomic variation in the three sunflower cultivars.