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目的探讨S-烯丙基巯基半胱氨酸(SAMC)对裸鼠胃癌模型移植瘤的抑制作用和相关机制。方法用裸鼠皮下胃癌SGC7901细胞注射法建立移植瘤模型,模型制作成功后将模型鼠分为对照组、SAMC低剂量组、SAMC高剂量组、5-FU组,每组6只。干预处理21d后观察各组肿瘤大小并计算每组肿瘤的瘤重、抑制率、肿瘤微血管密度及凋亡指数,免疫组织化学法检测各组肿瘤组织内血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达情况。结果与对照组相比,SAMC低剂量组、SAMC高剂量组、5-FU组移植肿瘤重量明显降低,抑瘤率分别为29.89%、51.85%和53.17%;微血管密度SAMC低剂量组、SAMC高剂量组、5-FU组均比对照组明显减少,分别为(11.78±7.49)%、(7.82±5.19)%、(7.91±4.37)%VS(16.21±8.52)%;凋亡指数比对照组明显升高,SAMC低剂量组、SAMC高剂量组、5-FU组分别为(11.82±1.60)%、(14.93±1.68)%和(15.92±1.72)%VS(4.36±1.55)%;免疫组织化学结果示VEGF表达量SAMC低剂量组、SAMC高剂量组、5-FU组均比对照组明显减少,平均光密度值(IOD)分别为11 240±1150、7 535±856、7 820±650 VS 18 500±1 522。结论 SAMC可明显抑制裸鼠原位肿瘤的生长,其机制可能通过抑制肿瘤新生血管的生成而诱导胃癌细胞凋亡。
Objective To investigate the inhibitory effect and its underlying mechanisms of S-allylmercaptocysteine (SAMC) on xenografted gastric cancer in nude mice. Methods The xenografted tumor model was established by injecting SGC7901 cells into the nude mice subcutaneously. The model mice were divided into control group, SAMC low dose group, SAMC high dose group and 5-FU group with 6 mice in each group. After intervention for 21 days, the tumor size of each group was observed and the tumor weight, inhibition rate, tumor microvessel density and apoptosis index were calculated. The expression of vascular endothelial growth factor (VEGF) in each group was detected by immunohistochemistry ) Of the expression. Results Compared with the control group, the tumor weight of SAMC low-dose group, SAMC high-dose group and 5-FU group were significantly decreased, the tumor inhibition rates were 29.89%, 51.85% and 53.17% respectively; the microvascular density SAMC low dose group, SAMC high (11.78 ± 7.49)%, (7.82 ± 5.19)% and (7.91 ± 4.37)% VS (16.21 ± 8.52)% respectively in the dose group and the 5-FU group. The apoptotic index in the 5-FU group was significantly lower than that in the control group (11.82 ± 1.60)%, (14.93 ± 1.68)% and (15.92 ± 1.72)% VS (4.36 ± 1.55)% respectively in SAMC low dose group, SAMC high dose group and 5-FU group. The chemical results showed that the expression of VEGF in SAMC low-dose group, SAMC high-dose group and 5-FU group were significantly lower than the control group, the average optical density values (IOD) were 11 240 ± 1150,7 535 ± 856,7 820 ± 650 VS 18 500 ± 1 522. Conclusion SAMC can significantly inhibit the growth of orthotopic tumor in nude mice, and its mechanism may induce apoptosis of gastric cancer cells by inhibiting angiogenesis.