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我们以前的工作表明糖皮质激素(GC)靶细胞上除存在经典的高亲和力、低容量GC受体(GR_H)外,尚存在低亲和力GC受体(GR_L)。GR_L可介导大剂量GC的作用。本文初步探讨了GR_L与GR_H在分子结构以及基因水平上的相互关系。利用两种抗大鼠肝GR_H单克隆抗体(MabN250:为抗GR_H N端免疫活性区的单抗,MabBuGR_1:为抗GR_H DNA结合区的单抗)制备Mab-Sepharose 4 B亲和层析柱,将[~3H]曲安缩酮(TA)与大
Our previous work demonstrated the presence of a low affinity GC receptor (GR_L) on glucocorticoid (GC) target cells in addition to the classic high affinity, low capacity GC receptor (GR_H). GR_L can mediate the effects of high-dose GC. In this paper, we initially discussed the relationship between GR_L and GR_H in molecular structure and gene level. The Mab-Sepharose 4 B affinity column was prepared with two anti-rat liver GR_H monoclonal antibodies (MabN250: mAb against the GR_H N-terminal immunoreactive region, MabBuGR_1: a monoclonal antibody against the GR_H DNA binding region) [~ 3H] triamterene (TA) with large