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[Objective] To establish a quantitative method for determination of quercetin content in Eriocaulon buergerianum,and determine the content of quercetin in E.buergerianum from different growing areas.[Method] High performance liquid chromatography(HPLC) coupled with four-channel UV-visible detector was used,C18 chromatographic column,mobile phase is the buffer solution of methanol and phosphoric acid(0.6%),the volume ratio of methanol and phosphoric acid(0.6%) was 52:48.Qualitative evaluation was done by retention time and four-channel UV spectrum at the same time,and the detection wavelength were 254,360,365,370 nm.Quantitative evaluation had done at the best sensitive wave of 254 nm.[Result] The linearity of quercetin was in the range of 5-30 μg/ml(r=0.999 8),the average recovery was 99.56%,the RSD was 2.30%.The content of quercetin in samples from different growing areas was in the range of 29.5 to 92.5 μg/ml.[Conclusion] The method is reliable,stable,feasible,and suitable for determination of quercetin content in E.buergerianum.Samples from different growing areas vary greatly in the content of quercetin.
[Objective] To establish a quantitative method for determination of quercetin content in Eriocaulon buergerianum, and determine the content of quercetin in E.buergerianum from different growing areas. [Method] High performance liquid chromatography (HPLC) coupled with four-channel UV-visible detector was used, C18 chromatographic column, mobile phase is the buffer solution of methanol and phosphoric acid (0.6%), the volume ratio of methanol and phosphoric acid (0.6%) was 52: 48.Qualitative evaluation was done by retention time and four -channel UV spectrum at the same time, and the detection wavelength were 254, 360, 365, 370 nm. Quantitative evaluation had done at the best sensitive wave of 254 nm. [Result] The linearity of quercetin was in the range of 5-30 μg / ml = 0.999 8), the average recovery was 99.56%, the RSD was 2.30%. The content of quercetin in samples from different growing areas was in the range of 29.5 to 92.5 μg / ml. [Conclusion] The method is reliable, stable, feasible, and suitable for dete rmination of quercetin content in E.buergerianum.Samples from different growing areas vary greatly in the content of quercetin.