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为确定表没食子儿茶素没食子酸酯(EGCG)对人支气管上皮Beas-2B细胞的抗氧化作用,本实验采用500μmol/L硫酸镍诱导建立细胞氧化损伤模型,通过测定细胞存活率、ROS生成量、谷胱甘肽水平及丙二醛含量,探讨不同剂量(2.5、5、10μmol/L)EGCG对硫酸镍诱导氧化损伤Beas-2B细胞的保护作用。结果表明,EGCG可以提高Beas-2B细胞的存活率,降低ROS生成量,提高GSH含量,降低MDA水平(P<0.05)。提示EGCG对Beas-2B细胞具有保护作用,其机制可能与减少细胞氧化损伤有关。
In order to determine the antioxidant effect of epigallocatechin-3-gallate (EGCG) on human bronchial epithelial cells Beas-2B, a cell oxidative injury model was established by using 500μmol / L nickel sulfate. Cell viability, ROS production , Glutathione (GSH) and malondialdehyde (MDA) contents in the Beas-2B cells induced by nickel sulfate were determined by different concentrations of EGCG (2.5, 5, 10μmol / L) The results showed that EGCG could increase the survival rate of Beas-2B cells, reduce the production of ROS, increase the content of GSH and decrease the level of MDA (P <0.05). Tip EGCG Beas-2B cells have a protective effect, the mechanism may be related to reducing cell oxidative damage.