真菌性角膜溃疡的确诊，有赖于从病变角膜上找到病原体（1），常用的方法有角膜刮片查找菌丝、真菌培养，偶尔有必要时行角膜活检加以证实（2）。但以往报告角膜刮片检菌法阳性率不高（3），真菌培养需要较长的时间，而角膜活检需行特殊染色，操作复杂。因此，探索一种快速且阳 The diagnosis of fungal corneal ulcer depends on finding the pathogen from the diseased cornea (1). Common methods include corneal scraping to find mycelium, culturing the fungus, and occasionally corneal biopsy (2). But in the past the positive rate of corneal smear detection method is not high (3), fungal culture takes a long time, and the corneal biopsy need special staining, the operation is complicated. Therefore, to explore a fast and positive