论文部分内容阅读
近二十年来,由于染色体研究技术的不断改进,在染色体核型分析等方面作了大量的工作并有详细的报道,在实用和理论上都有重要的发展。研究由于电离辐射引起的染色体畸变,已作为衡量放射损伤的一种生物剂量仪而应用。更因发现染色体分带技术,为染色体的研究开辟了新的前景。因此这一研究已成为医学上一项日益重要的工作。但是,培养细胞一般所使用的199培养液或益格氏培养液等,不但原料昂贵,而且配制手续复杂,灭菌困难,保存不易,以致这种染色体研究工作难以较普遍地开展。我们考虑,血液中天然具有血细胞生存所需的完善条件和发育所需的营养成分,血细胞在本身的血液中进行培养理应获得正常的生存和发育。依据这种想法,我们创用了以血养血的方法,经过充分的试验,效果良好。同时,从取血到制片各个环节的工作我们都作了改进,制出了染色体分散良好的标本,并已应用于临床诊断工作。现将方法概述于下。
In the past 20 years, due to the continuous improvement of chromosome research techniques, a great deal of work has been done on chromosome karyotype analysis and has been reported in detail. Both of them have important developments in both practical and theoretical aspects. Studying chromosomal aberrations due to ionizing radiation has been used as a biological dosimeter for measuring radiation damage. More because of the discovery of chromosome banding technology, opened up a new prospect for the study of chromosomes. Therefore, this research has become an increasingly important medical work. However, the culture medium used for culturing cells, such as 199 medium or medium of Iges, not only is expensive, but also has complicated preparation procedures, difficult sterilization and poor preservation, making it difficult to carry out such chromosome research. We consider that the blood naturally has the perfect conditions for the survival of the blood cells and the nutrients needed for development. The blood cells should be cultured normally in their own blood to obtain normal survival and development. Based on this idea, we have created a blood-based method, after sufficient testing, the effect is good. At the same time, we have made improvements from the blood collection to all aspects of filmmaking and have produced well-dispersed samples of chromosomes and have been used in clinical diagnosis. The method is summarized below.