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目的回顾性分析8例抗-P1标本,为抗-P1引起的输血相容性检测困难提供解决方案。方法血型鉴定采用强生Auto VUE玻璃珠卡式法,正反定型不一致样本用试管法进行复查;抗体筛查用强生Auto VUE抗人球卡进行检测,阳性样本用Diana抗人球卡做抗体鉴定;试管法鉴定被检者红细胞上是否存在P1抗原,必要时检测反定试剂红细胞是否存在P1抗原;统计8例标本配血相合率。结果 8例抗筛阳性标本均导致ABO正反定型不符,抗体鉴定结果均为抗-P1,配血相合率为14%-67%。结论抗-P1引起正反定型不一致时,应使用P1抗原阴性试剂红细胞进行血型反定型,以确保结果的准确性。
Objective To retrospectively analyze 8 anti-P1 samples to provide a solution to the difficulties of detecting the compatibility of transfusion induced by anti-P1. Methods The blood type was identified by Johnson & Johnson Auto VUE glass beads method. Inconsistent positive and negative samples were examined by in vitro test. Antibody screening was performed with Johnson & Johnson Auto VUE anti-human card. Positive samples were identified by Diana anti- Test tube method to identify the presence or absence of P1 antigen on the erythrocytes, if necessary, detect the presence or absence of P1 antigen in the erythrocytes; statistics of 8 specimens with blood compatibility. Results The results of positive and negative ABO assay in all 8 cases of positive anti-screening showed that the results of antibody identification were anti-P1 and the matching rate of blood was 14% -67%. Conclusion Anti-P1 caused by inconsistent positive and negative stereotypes, the red blood cells should be used P1 negative antigen-type anti-type to ensure the accuracy of the results.