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在食用菌的代料栽培中,特别是在生料开放式栽培中,杂菌的孳生蔓延对生产危害很大。为了寻找抗污染药物,1987年春,我们在生料栽培香菇中用绿霉A_(12)浸出液作抑菌剂防霉,收到了良好的效果,现初报如下:材料和方法(一)供试材料菌种为香菇7402和Cr02两菌株。抑菌剂设:A.农用多菌灵;B.高锰酸钾;C.绿霉A_(12)浸出液。绿霉A_(12)是从木材上分离所得,将A_(12)接种在麸皮培养基中(麦麸79%、木屑20%、糖1%,pH4～5.5),恒温培养20天,至培养瓶中绿霉长满瓶、瓶壁反青时,将其挖出按3:1的量加水,在电炉上煮沸30分钟,然后用四层纱布过滤,其滤液便为A_(12)浸出液。
In the food cultivation of edible fungi, especially in the raw material open cultivation, the spread of miscellaneous bacteria on the production of a great harm. In order to search for anti-pollution drugs, we used mold green leachate (A_ (12)) as antimicrobial to prevent mildew in the spring of 1987 and received good results. The first report is as follows: Materials and Methods Material strains of mushrooms 7402 and Cr02 two strains. Bacteriostatic agents: A. agricultural carbendazim; B. potassium permanganate; C. green mold A_ (12) leachate. The green mold A_ (12) was isolated from wood and A_ (12) was inoculated into wheat bran medium (wheat bran 79%, sawdust 20%, sugar 1%, pH4-5.5) When the flask is covered with bottle of green mold and the wall of the flask is anti-green, it is dug into the flask with water in an amount of 3: 1, boiled in an electric cooker for 30 minutes and then filtered through a four-layer gauze. The filtrate is A_ (12) .