论文部分内容阅读
背景:研究证实,以生物材料为中心的感染细菌临床株致病力与其在中心静脉导管材料表面形成细菌生物膜的能力呈正相关。目的:分析肺癌患者中心静脉导管相关表皮葡萄球菌icaA、icaDmRNA表达及外周血转化生长因子β1水平与细菌生物膜形成的关系。方法:种属鉴定相关性血流感染肺癌患者表皮葡萄球菌类型后行细菌基因组DNA抽提,PCR法检测生物膜形成相关基因icaA、icaDmRNA表达及生物膜表型。酶联免疫吸附试验检测相关性血流感染与未感染肺癌患者血清转化生长因子β1水平。结果与结论:相关性血流感染肺癌患者表皮葡萄球菌操纵子icaA、icaD基因表达与生物膜形成呈正相关(P<0.01),且表皮葡萄球菌生物膜阳性患者外周血转化生长因子β1水平较无相关性血流感染肺癌患者高(P<0.05)。表明置入中心静脉插管引起表皮葡萄球菌感染icaA、icaD基因表达阳性肺癌患者较易形成细菌生物膜,外周血高水平转化生长因子β1对细菌生物膜形成有积极作用。
BACKGROUND: Studies confirm that the virulence of clinical isolates of infectious bacteria with biomaterials is positively correlated with their ability to form bacterial biofilms on the surface of central venous catheters. Objective: To analyze the relationship between icaA and icaD mRNA expression in central venous catheter-associated Staphylococcus epidermidis and the level of peripheral blood transforming growth factor-β1 and the formation of bacterial biofilm in patients with lung cancer. Methods: Bacterial genomic DNA was extracted from Staphylococcus epidermidis in patients with lung cancer after blood-borne infection of species identification. The biofilm-related gene icaA, icaD mRNA and biofilm phenotype were detected by PCR. Enzyme linked immunosorbent assay was used to detect the serum levels of TGF-β1 in patients with and without pulmonary infection. RESULTS AND CONCLUSION: There was a positive correlation between icaA and icaD gene expression and biofilm formation in patients with lung cancer associated with bloodstream infection (P <0.01), and the levels of TGF-β1 in peripheral blood from patients with positive S. epidermidis biofilms were significantly lower than those without Correlated bloodstream infections were associated with lung cancer (P <0.05). These results suggest that it is easier to form bacterial biofilms in patients with icaA and icaD gene positive staphylococcal infections caused by central venous catheterization. High levels of transforming growth factor-β1 in peripheral blood have a positive effect on bacterial biofilm formation.