目的:建立新的人视网膜母细胞瘤细胞株,对其进行冻存和复苏,为临床、科研提供Rb细胞库。方法:用精确优化的取材,不经离心,直接通过洗涤吹打细胞后接种,并根据慢冻速融的细胞冻存原则,定期收集建株的Rb_(70)细胞作液氮冻存及复苏。结果:成功建立SO-Rb_(70)细胞株,共冻存8批细胞,进行了6次复苏实验,4次获成功。结论:经液氮冻存的人Rb_(70)细胞,复苏后生长良好,保持了细胞原有的生物活性及细胞特征,为Rb的深入研究提供了丰富的实验研究材料。眼科学报1998;14:80—82。 OBJECTIVE: To establish a new human retinoblastoma cell line which is cryopreserved and resuscitated to provide Rb cell bank for clinical and scientific research. Methods: The cells were directly inoculated by washing after being centrifuged without using centrifugation, and the frozen-thawed cells were collected regularly for cryopreservation and resuscitation. RESULTS: SO-Rb_ (70) cell lines were successfully established, and 8 batches of cells were cryopreserved. Six resuscitation experiments were performed, and 4 were successful. CONCLUSION: Human Rb 70 cells, which are frozen in liquid nitrogen, grow well after resuscitation and maintain the original biological activity and cell characteristics of the cells, providing abundant experimental research materials for Rb. Ophthalmic Science 1998; 14: 80-82.