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目的探讨放射免疫显像(RII)在食管癌分期的应用。方法1LSAB法测定组织切片和抗人食管鳞癌单抗G9的反应;2125I标记G9,在荷食管癌裸鼠腹腔注射125IG9后的连续3d内,测定各主要组织、器官的放射性;3食管鳞癌术前经纤维食管镜在原发灶周围黏膜下注射131IG9后行RII;4对清扫淋巴结行放射性测定。结果1LSAB显示癌原发灶和转移淋巴结呈阳性,正常食管和淋巴结阴性;2125IG9在裸鼠体内分布显示,肿瘤组织的放射性计数均明显高于其它器官/组织;3镜下注药48h时RII在食管旁纵隔、贲门旁、胃小弯旁出现散在的细点状放射性浓集,浓集区域所清扫的淋巴结中,包含有转移淋巴结;无浓集区域,未发现转移淋巴结;4全部有转移淋巴结的放射性比活度均比无转移者高,且均数高出两倍多。结论1单抗G9能选择性定位于食管鳞癌细胞膜;2125IG9在荷食管癌裸鼠有肿瘤组织导向作用,显像效果良好;3用131IG9在食管鳞癌行RII,对转移淋巴结有定位作用可作为cTNM分期的选择手段。
Objective To investigate the application of radioimmunoimaging (RII) in the staging of esophageal cancer. Methods 1LSAB method was used to determine the response of tissue section and anti-human esophageal squamous cell carcinoma monoclonal antibody G9. 2125I-labeled G9 was used to measure the radioactivity of major tissues and organs within 3 days after intraperitoneal injection of 125IG9 in nude mice bearing esophageal cancer. 3 Esophageal squamous cell carcinoma Preoperative fiberoptic esophagoscopy was used to perform RII after submucosal injection of 131IG9 around the primary lesion; 4 radioactivity measurements were performed on lymph node dissection. Results 1LSAB showed positive tumor and lymph node metastasis, normal esophageal and lymph node were negative; 2125IG9 showed distribution in nude mice, radioactivity count of tumor tissue was significantly higher than other organs/tissue; 3 RII was at 48h after microscopic injection. Sparse fine-spotted radioactivity concentration around the esophageal mediastinum, parapneousus, and minor gastric curvature. Lymph nodes cleared by the concentration region contained metastatic lymph nodes; no concentration regions were found, no metastatic lymph nodes were found; 4 all had metastatic lymph nodes. The specific activity of radioactivity was higher than those without metastasis, and the mean was more than twice as high. Conclusion 1 Monoclonal antibody G9 can be selectively localized in the esophageal squamous cell carcinoma membrane; 2125IG9 is a tumor-directed effect in nude mice bearing esophageal cancer, and the imaging results are good; 3 RII in esophageal squamous cell carcinoma with 131IG9 can locate the metastatic lymph nodes. As a choice of cTNM stages.