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目的:建立通滞苏润江胶囊中番泻苷A、B的含量测定方法。方法:采用HPLC法对通滞苏润江胶囊中的番泻苷A、B含量进行测定,SinoChrom ODS BP色谱柱(250 mm×4.6 mm,5μm),流动相乙腈-5 mmol·L-1四庚基溴化铵的醋酸-醋酸钠缓冲液(pH5.0)(1→10)(37∶63),流速:1.0 ml·min-1,柱温:40℃,检测波长:340 nm。结果:番泻苷A进样量在41.971~2 098.552 ng的范围内线性关系良好(r=0.999 9),平均回收率为99.52%(RSD=1.39%,n=6);番泻苷B进样量在42.781~2 139.046 ng的范围内线性关系良好(r=0.999 9),平均回收率为99.36%(RSD=1.45%,n=6)。结论:方法简便快捷,适用于该制剂中番泻苷A、B的含量测定。
OBJECTIVE: To establish a method for the determination of sennoside A and B in Tongrunsu Runjiang capsule. Methods: The contents of sennoside A and B in Tongli SuRunJiang capsule were determined by HPLC. SinoChrom ODS BP column (250 mm × 4.6 mm, 5 μm), mobile phase acetonitrile-5 mmol·L-1 tetraheptyl Ammonium bromide buffer (pH 5.0) (1 → 10) (37:63), flow rate: 1.0 ml · min-1, column temperature: 40 ℃, detection wavelength: 340 nm. Results: Sennoside A injection volume in the range of 41.971 ~ 2 098.552 ng good linear relationship (r = 0.999 9), the average recovery was 99.52% (RSD = 1.39%, n = 6); Sennoside B into The linear range was 42.781 ~ 2 139.046 ng (r = 0.999 9) with an average recovery of 99.36% (RSD = 1.45%, n = 6). Conclusion: The method is simple and quick and suitable for the determination of sennoside A and B in this preparation.