论文部分内容阅读
采用造血祖细胞体外培养和造血生长因子检测等实验血液学技术,研究三七总皂甙对小鼠红系祖细胞增殖调控的生物学机理.结果表明:三七总皂甙对正常或骨髓抑制一贫血模型小鼠的红系祖细胞增殖有明显促进作用;三七总皂甙亦可提高阿糖胞苷所致祖细胞的“自杀”率;经三七总皂甙诱导制备的脾细胞、L细胞(成纤维细胞株)培养上清液对红系祖细胞的增殖具有较高刺激活性.该文提示三七总皂甙可能通过诱导造血微环境中的成纤维细胞、淋巴细胞等分泌较高活性的红系造血调控因子和/或协同造血生长因子,以促进红系祖细胞增殖分化,这或许是三节“补气、活血”的生物学机理之一.
Using experimental hematopoietic techniques such as hematopoietic progenitor cell culture in vitro and hematopoietic growth factor assays, the biological mechanism of panax notoginseng saponins proliferation in mouse erythroid progenitor cells was studied. The results showed that: Panax notoginseng saponins normal or myelosuppression anemia The proliferation of erythroid progenitor cells in model mice has a significant promotion effect; panax notoginseng saponins can also increase the “suicide” rate of cytarabine-derived progenitor cells; and spleen cells and L cells induced by Panax notoginseng saponins can be obtained. Fibroblast cell culture supernatants have high stimulatory activity on the proliferation of erythroid progenitor cells. This article suggests that Panax notoginseng saponins may secrete more active erythroids by inducing fibroblasts, lymphocytes, etc. in hematopoietic microenvironment. Hematopoietic regulatory factors and/or synergistic hematopoietic growth factors are used to promote the proliferation and differentiation of erythroid progenitor cells. This may be one of the biological mechanisms of the three sections of “supplement gas and blood circulation.”