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ＡＲＳ２，ＡＲＳ７，ＡＲＳ８是从水稻珍汕９７Ａ不育系核ＤＮＡ中克隆的一组在酵母细胞中能起始 ＤＮＡ复制的片段．其中ＡＲＳ８是单拷贝顺序，而ＡＲＳ２，ＡＲＳ７却是高度重复顺序．将带有ＡＲＳ２，ＡＲＳ７，ＡＲＳ８的质粒用低压脉冲电泳法导入水稻部分酶解小细胞团中，经浅层培养，辅板，获得愈伤组织，Ｋｍ筛选获得抗性愈伤组织．抽提抗性愈伤组织ＤＮＡ，经Ｓｏｕｔｈ－ｅｒｎ点杂交证实ＡＲＳ２，ＡＲＳ７分别有３．２％，３．６％愈伤组织导入了外源基因，较对照高１倍和 １． ３倍．ＨｉｎｄⅢ酶解抗性愈伤组织 ＤＮＡ，琼脂糖凝胶电泳分离，转膜，Ｓｏｕｔｈｅｒｎ杂交，发现ＡＲＳ２和 ＡＲＳ７呈多位点整合．
ARS2, ARS7 and ARS8 are clones that can initiate DNA replication in yeast cells cloned from the nuclear DNA of Zhenshan 97A CMS line. Among them ARS8 is a single copy sequence, while ARS2 and ARS7 are highly repetitive sequences. The plasmids with ARS2, ARS7 and ARS8 were introduced into partially digested rice cells by low voltage pulse electrophoresis. After the supernatant was cultured in shallow layer, the callus was obtained and the callus was screened by Km to obtain the resistant callus. The DNA of resistant callus was extracted. The Southern blot analysis showed that the exogenous genes of ARS2 and ARS7 were 3.2% and 3.6%, respectively. 3 times. Hind Ⅲ enzymatic digestion of the callus DNA, agarose gel electrophoresis separation, transfection, Southern hybridization and found ARS2 and ARS7 was multi-site integration.