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作者将解冻后的人新鲜冰冻血浆（FFP）76ml与1ml人类免疫缺陷症病毒（HIV）浓缩物混合,至HIV-l终浓度为10~6半数组织培养感染量（TCID_(50)）/ml。然后以3份上述制品作如下处理:（1）暗处理对照:制品中加入1μM亚甲蓝（MB）,室温下于暗处培育;（2）可见光照射处理对照:不加MB但作光照处理;（3）MB/光照处理试验:含病毒血浆在适宜的生产条件下加1μM MB并作光照处理。为控制光照处理前后血浆本身可能有的细胞毒素,将3份无病毒的血浆样品各5ml作同样
The authors mixed 76 ml of thawed frozen plasma (FFP) with 1 ml of human immunodeficiency virus (HIV) concentrate to a final HIV-1 concentration of 10 to 6 tissue culture inoculum (TCID 50) / ml . Then 3 parts of the above products were treated as follows: (1) dark treatment control: 1μM methylene blue (MB) was added into the product and incubated in the dark at room temperature; (2) visible light irradiation control: no MB but light treatment ; (3) MB / light treatment test: Virus-containing plasma was treated with 1 μM MB under suitable production conditions. To control the possible presence of cytotoxins in the plasma before and after light treatment, 5 ml of 3 virus-free plasma samples