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目的 研究原发性肝细胞性肝癌 (HCC)细胞凋亡的发生情况及与凋亡相关的 p53基因的表达 ,探讨p53基因与细胞凋亡的关系。方法 以TUNEL法检测HCC细胞凋亡的发生情况 ,免疫组化SABC法检测p53基因的表达。结果 34例HCC平均凋亡指数为4.16 %±0.70% ,有18例p53蛋白阳性 ,凋亡指数的高低与p53蛋白的阳性率有关 ,p53蛋白阳性者凋亡指数低 ;正常肝组织平均凋亡指数为0.51%±0.09 % ,无 p53蛋白阳性者。结论 HCC患者中存在肿瘤细胞增殖和死亡调控的异常 ,其细胞凋亡的发生可能与p53基因失控有关
Objective To study the apoptosis of hepatocellular carcinoma (HCC) and the expression of p53 gene associated with apoptosis, and to explore the relationship between p53 gene and apoptosis. Methods TUNEL method was used to detect the apoptosis of HCC cells. The expression of p53 gene was detected by immunohistochemical SABC method. Results The average apoptotic index of 34 HCC patients was 4.16% ± 0.70%, and 18 cases were positive for p53 protein. The apoptosis index was correlated with the positive rate of p53 protein, and the apoptosis index of p53 protein positive was lower. The average apoptosis rate of normal liver tissue Index was 0.51% ± 0.09%, no p53 protein positive. Conclusion HCC patients have tumor cell proliferation and death control abnormalities, and the occurrence of apoptosis may be related to the loss of p53 gene