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目的了解2015-2016年北京地区儿童肺炎支原体(MP)耐药情况及耐药机制分析。方法采集2015-2016年首都医科大学附属北京朝阳医院儿科收治的,具有发热、咳嗽或咽痛等呼吸道感染症状之一的,门诊和住院的89例患儿的呼吸道咽拭子标本,进行MP培养与药敏试验,采用巢式PCR扩增法鉴定MP耐药位点。结果 53例MP阳性,感染率为59%,18株为敏感株,耐药率66%,32株存在23SrRNA结构域Ⅴ区A2063G点突变。3株2063、2064位点均有突变。结论北京地区肺炎支原体感染率高,对大环内酯类抗生素耐药率上升趋势,耐药机制主要为23SrRNA结构域Ⅴ区点突变。
Objective To investigate the multidrug resistance and drug resistance mechanisms of children with Mycoplasma pneumoniae (MP) in Beijing from 2015 to 2016. Methods A total of 89 throat swab samples from outpatients and hospitalized patients with respiratory symptoms of fever, cough or sore throat were collected from pediatric department of Beijing Chaoyang Hospital affiliated to Capital Medical University from 2015 to 2016 for MP culture And drug sensitivity test, using nested PCR amplification method to identify MP resistant sites. Results Fifty-three cases were positive for MP, the infection rate was 59%. 18 strains were sensitive and the drug resistance rate was 66%. 32 strains had A2063G point Ⅴ in the region of 23S rRNA. Three strains 2063,2064 sites have mutations. Conclusions The prevalence of Mycoplasma pneumoniae infection in Beijing is high, and the resistance rate to macrolide antibiotics is on the rise. The mechanism of drug resistance mainly consists of point Ⅴ mutation in 23S rRNA domain.