为分析多发性硬化症 (MS )患者发作期淋巴细胞亚群及给予甲基强的松龙 (MP )治疗后的变化 ,流式细胞仪测定 2 6例处于复发期MS患者外周血 (PB )和脑脊液 (CSF )及 8例MS患者予MP治疗后PB淋巴细胞CD3+ 、CD4 + 、CD8+ 、CD4 5RA+ 、CD4 + /CD4 5RA+ 、CD4 + /CD2 9+ 、CD19+ 、CD5 + /CD19+ 的百分率。结果发现MS患者PB中CD8+ 、CD4 5RA+ 和CD4 + /CD4 5RA+ 百分率降低 ,CD4 + /CD2 9+ 百分率和CD4 + /CD8+ 比值升高 ;CSF中CD3+ 、CD4 + 、CD4 + /CD2 9+ 百分率和CD4 + /CD8+ 比值高于PB ;淋巴细胞亚群与临床伤残程度和距此次发作的时间无关 ;MP治疗不影响PB淋巴细胞亚群变化。表明MS患者淋巴细胞通过血脑屏障有选择性 ,淋巴细胞亚群的变化在MS发病机制中起作用 In order to analyze the change of lymphocyte subsets in patients with multiple sclerosis (MS) and the treatment of methylprednisolone (MP), flow cytometry was used to detect the peripheral blood (PB) And cerebrospinal fluid (CSF) in 8 MS patients and the percentage of CD3 +, CD4 +, CD8 +, CD4 5RA +, CD4 + / CD4 5RA +, CD4 + / CD2 9+, CD19 +, CD5 + / CD19 + of PB lymphocytes after MP treatment. The results showed that the percentage of CD8 +, CD4 + RA + and CD4 + / CD4 + RA + in PB of MS patients was decreased, and the percentage of CD4 + / CD2 + and CD4 + / CD8 + increased. The percentage of CD3 +, CD4 +, CD4 + / CD2 + The ratio of CD4 + / CD8 + was higher than that of PB. The level of lymphocyte subsets and clinical disability had no relation with the duration of the attack. MP treatment did not affect the changes of PB lymphocyte subsets. Indicating that MS patients with lymphocytes through the blood-brain barrier is selective, changes in lymphocyte subsets play a role in the pathogenesis of MS