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利用全套噬菌体抗体表面展示技术，绕过杂交瘤技术，从重组人G-CSF免疫的小鼠脾淋巴细胞中提取总RNA，反转录成cDNA后，用抗体可变区PCR混合引物进行全套抗体重、轻链可变区（VH和VL）基因的扩增。经重叠延伸反应，在体外随机装配成单链抗体（ScFv）。将其克隆至噬菌粒载体pCANTAB5E中，电转化含SupE的E.Coli菌株，以辅助噬菌体M13K07超感染噬菌粒文库，构建成全套ScFv表面展示文库。为利用亲和富集筛选技术，获得具有G-CSF结合活性的完整重组噬菌粒克隆奠定了基础。
Total RNA was extracted from splenic lymphocytes of mice immunized with recombinant human G-CSF by using a full set of phage antibody surface display technology and bypassing the hybridoma technique. After reverse transcription into cDNA, the complete set of antibodies Body weight, light chain variable region (VH and VL) gene amplification. After overlapping extension reaction, they were randomly assembled into single chain antibody (ScFv) in vitro. Cloned into the phagemid vector pCANTAB5E and electroporated into SupE-containing E.Coli strain to supplement the phagemid M13K07 super-infected phagemid library to construct a complete set of ScFv surface display library. It provides the basis for obtaining complete recombinant phagemid clone with G-CSF binding activity by using affinity enrichment screening technology.