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目的 探讨Fas FasL系统介导的细胞凋亡在肾综合征出血热 (HFRS)致病机理中的作用。方法 采用SABC免疫组化法检测HFRS病人外周血淋巴细胞Fas FasL的表达 ;采用ELISA法测定HFRS病人血清sFas sFasL的浓度。结果 HFRS病人发热期、低血压休克期、少尿期及多尿期外周血淋巴细胞Fas的表达明显增强 ,与正常对照组比较差异有极显著性 (P <0 .0 0 1) ;发热期、低血压休克期及少尿期淋巴细胞FasL的表达明显增强 ,与正常对照组比较差异有极显著性 (P <0 .0 0 1) ;HFRS病人各期血清sFas的浓度增高 ,与正常对照组比较差异有显著性 (P <0 .0 1) ,其中少尿期sFas的浓度最高 ,与其它各期比较差异有显著性 (P <0 .0 1) ;发热期与低血压休克期血清sFasL的浓度增高 ,与其它各期及正常对照组比较差异有显著性 (P <0 .0 1) ;淋巴细胞Fas及FasL的表达经直线相关分析(r=0 .885 5 ,P <0 .0 0 1) ,呈高度正相关 ;淋巴细胞FasL的表达与血清sFasL浓度经直线相关分析(r=0 .480 3,P <0 .0 1) ,呈高度正相关。结论 HFRS的致病过程中存在Fas FasL介导的细胞凋亡 ,sFas浓度的高低与机体损害的程度有关 ;淋巴细胞FasL的高表达及血清sFasL的浓度增高 ,由此而引起的细胞凋亡是机体清除病毒的主要机制之一
Objective To investigate the role of Fas FasL system-mediated apoptosis in the pathogenesis of hemorrhagic fever with renal syndrome (HFRS). Methods The expression of Fas FasL in peripheral blood lymphocytes of patients with HFRS was detected by SABC immunohistochemical method. The serum concentration of sFas sFasL in HFRS patients was measured by ELISA. Results The expression of Fas in peripheral blood lymphocytes in fever, hypotensive shock, oliguria and polyuria in HFRS patients was significantly higher than that in controls (P <0.01). The incidence of fever , The expression of FasL in the hypothalamus and oliguria phase lymphocytes was significantly increased, the difference was significant compared with the normal control group (P <0.01); the concentration of sFas in each stage of HFRS patients was increased, (P <0.01). The concentration of sFas in the oliguria period was the highest, which was significantly different from the other periods (P <0.01). The difference between the febrile sera and the sera in hypotension The concentration of sFasL increased compared with other phases and normal control group (P <0.01). The expression of Fas and FasL in lymphocytes was analyzed by linear correlation analysis (r = 0.885 5, P <0. There was a positive correlation between the expression of FasL and the concentration of sFasL in lymphocytes by linear correlation analysis (r = 0.4880, P <0.01). Conclusion Fas FasL-mediated apoptosis exists in the pathogenesis of HFRS. The concentration of sFas is related to the degree of damage to the body. The high expression of FasL in lymphocytes and the increase of serum sFasL concentration induce the apoptosis of One of the main mechanisms of the body to clear the virus