LRP16是我们应用甲基化敏感的限制性界标基因组扫描 (restrictionlandmarkgenomicscanning ,RLGS)技术时发现的一个与白血病复发相关的新基因。为钓取这一新基因的全长cDNA ,本实验采用了cDNA末端快速扩增法 (rapidamplificationofcDNAend ,RACE)。通过对RACE法若干步骤进行优化 ,克隆得到了LRP16基因cDNA的 5′ 与 3′ 非翻译区 ,进而获得其全长及完整的开放阅读框架 ,并作为新基因被GenBank收录。上述结果表明 ,RACE技术是钓取未知基因全长cDNA敏感而快速的方法 ,尤其是对 5′ 及 3′ 非翻译区的钓取更具有实用性 LRP16 is a novel gene associated with the relapse of leukemia we found using methylation-sensitive restriction landmarkgenomicscanning (RLGS) technique. To capture the full-length cDNA of this new gene, rapid amplification of cDNA ends (RACE) was used in this experiment. By optimizing several steps of RACE, the 5 ’and 3’ untranslated regions of cDNA of LRP16 gene were cloned, and the full-length and complete open reading frame was obtained, which was then reported as a new gene in GenBank. The above results indicate that RACE technology is a sensitive and rapid method to capture the full-length cDNA of unknown genes, especially for the 5 ’and 3’ untranslated regions