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目的:研究半夏超临界CO2乙醇萃取物对不同惊厥动物模型的对抗作用,并探讨其作用机制。方法:采用最大电休克(MES)和戊四唑惊厥模型(MET),以托吡酯(TPM)为阳性对照,观察其抗惊厥作用;建立大鼠皮层定位注射青霉素点燃模型,观察其灌胃给药对惊厥行为和脑电图的影响。结果:半夏超临界CO2萃取物对MES模型有对抗作用,且量效呈正相关性。可延长MET惊厥潜伏期(P<0.01),对抗MET发作。对青霉素点燃模型亦产生对抗作用,可延长痫性发作潜伏期、减轻发作程度,延长痫性放电的潜伏期,减少痫波发放频率,减小放电最高波幅,与模型组比较,均具有显著性差异(P<0.05,P<0.01)。结论:半夏超临界CO2乙醇萃取物可剂量依赖性对抗小鼠最大电休克惊厥,对抗戊四唑惊厥,也能对抗大鼠皮层定位注射青霉素点燃发作,其抗惊厥作用性质和托吡酯相似,而抗青霉素点燃发作较托吡酯强。
Objective: To study the antagonistic effect of ethanol extracts of Pinellia by supercritical CO2 on animal models of different convulsions and to explore its mechanism. Methods: The anticonvulsant effect was observed by top electrocaping (MES) and pentylenetetrazole (MET) model and topiramate (TPM) as positive control. The cortical injection model of penicillin was established to observe the effect of intragastric administration Impact on convulsions and electroencephalography. Results: Pinellia supercritical CO2 extract had antagonistic effect on MES model, and the dose-effect showed a positive correlation. Prolonged MET latency (P <0.01) against MET onset. It also antagonized the model of penicillin, prolonged the latency of seizure, reduced the degree of seizure, prolonged the latency of epileptic discharge, reduced the frequency of discharge of epileptic seizure, and reduced the maximum amplitude of discharge. Compared with the model group, there was significant difference P <0.05, P <0.01). Conclusion: Pinellia supercritical CO2 ethanol extract can antagonize the maximal electroshock convulsion in mice in a dose-dependent manner, antagonize the pentylene tetrazolium convulsions, and also can antagonize the focal cortical injection of penicillin in rats. The anticonvulsant effect is similar to that of topiramate Anti-penicillin ignition is more powerful than topiramate.