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用荧光测量方法，对被认为可作本底水样的几种水体进行了荧光特性的测量研究，确认了即时处理的Milli-Q水是无荧光效应的高纯度水，可作为本研究的本底水样。同时，对海水黄色物质Milli-Q水溶液、市售草炭腐殖质Milli－Q水溶液、青岛近岸海水和褐藻培养液样品进行了光谱特性测量研究。结果表明，海水黄色物质和培养液的黄色物质的激发光谱峰值波长在340nm附近、荧光光谱峰值波长在435nm附近；而草炭腐殖质的激发峰值波长在320nm附近，荧光峰值波长在440nm附近。测量得出了海水黄色物质Milli-Q水溶液的荧光强度与其浓度的关系曲线。其最低检测浓度高达1×10－9／ml以上。同时也测量得出了青岛近岸海水样品不同稀释度的稀释液与对应的荧光强度的良好正相关关系。用荧光法测量得出青岛近岸海水的黄色物质浓度为3．2×10－7g／ml。
Fluorescence measurement was used to measure the fluorescence characteristics of several water bodies that were considered as background water samples. It was confirmed that the immediately-treated Milli-Q water is a non-fluorescent high-purity water that can be used as the basis of this study Bottom water samples. At the same time, the spectroscopic properties of seawater yellow material Milli-Q aqueous solution, commercial peat humic Milli-Q aqueous solution, Qingdao coastal seawater and brown algae culture medium were studied. The results showed that the peak wavelength of excitation spectrum of yellow matter in seawater yellow matter and culture solution was around 340nm and the peak wavelength of fluorescence spectrum was near 435nm. The excitation peak wavelength of peat humus was near 320nm, and the fluorescence peak wavelength was around 440nm. The fluorescence intensity of the aqueous solution of Milli-Q, which is a yellow substance of seawater, is plotted against its concentration. The lowest detection concentration of up to 1 × 10-9 / ml or more. At the same time, a good positive correlation was also obtained between the dilutions of different dilutions and the corresponding fluorescence intensities of Qingdao coastal samples. The yellow substance concentration of Qingdao coastal seawater measured by fluorescence method was 3.2 × 10-7g / ml.