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目的了解南通地区乙型肝炎病毒基因分型情况及其临床意义。方法用PCR法扩增HBV PreS基因并测序,共分析慢性无症状携带者(ASC)、慢性活动性肝炎患者(CAH)和肝癌(HCC)患者血清86份。用C lustalx1.8软件比对,用MEGA3.1作树分型,同时测定样品HBeAg和HBV-DNA。结果南通地区存在HBV B型、C型和B/C混合感染型,其中C型比例最高,占67.44%,而B型和B/C混合感染型均占16.28%。C型患者的血清HBeAg阳性率和HBV-DNA浓度显著高于其他基因型(p<0.05)。结论南通地区存在HBV B型、C型以及B+C混合型,C型为优势型;C型患者的病毒复制最为活跃。
Objective To understand the genotypes of hepatitis B virus in Nantong area and its clinical significance. Methods HBV PreS gene was amplified by PCR and sequenced. A total of 86 serum samples from patients with chronic asymptomatic carriers (ASC), chronic active hepatitis (CAH) and hepatocellular carcinoma (HCC) were analyzed. C lustalx1.8 software alignment, MEGA3.1 for tree type, simultaneous determination of samples HBeAg and HBV-DNA. Results There were HBV B, C and B / C mixed infections in Nantong area, of which C was the highest, accounting for 67.44%, while B and C were 16.28%. The serum HBeAg positive rate and HBV-DNA concentration of type C patients were significantly higher than those of other genotypes (p <0.05). Conclusions There are HBV B type, C type and B + C mixed type in Nantong region, while C type is the predominant type. In C type, the virus replication is the most active.