体外培养乳鼠心肌细胞是药理学研究中的有用工具,尤其在药物—受体作用的研究中可进行直接的受体结合研究,具有独特的优点。[~3H]-二苯羟乙酸奎宁酯(~3H-QNB)是一种强效的M-胆碱能拮抗剂,能与M-受体紧密结合,具有很高的特异性。它可作为放射性配基进行受体结合研究,本实验应用放射配基受体结合法,以放射自显影技术直接观察培养乳鼠心肌细胞中的M-胆碱能受体。 In vitro culture of cardiomyocytes in neonatal rats is a useful tool in pharmacological research, especially in the study of drug-receptor interaction, which has the unique advantages of direct receptor binding. [~ 3H] -benzimidyl behenate (~ 3H-QNB) is a potent M-cholinergic antagonist that binds tightly to the M-receptor with high specificity. It can be used as a radioligand receptor binding study, the experiment using radioligand receptor binding method to autoradiography directly observed cultured neonatal cardiomyocytes in the M-cholinergic receptors.