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利用B1.7cDNA特异性引物、RT-PCR技术从新鲜淋巴瘤组织中扩增得到一与标准型B7.1片段长度不同的B7.1分子。进一步将该片段插入真核表达载体PSVL中，转染细胞得到了较高水平的表达。免疫组化研究结果证实抗B7.1单抗可与该片段编码的蛋白结合，提示该cDNA片段为B7.1抗原的拼接异构体。
Using B7.1 cDNA specific primers, RT-PCR technique was used to amplify a novel B7.1 molecule with a length different from that of the standard B7.1 fragment from fresh lymphoma tissue. The fragment was further inserted into the eukaryotic expression vector PSVL and the transfected cells obtained a higher level of expression. The result of immunohistochemistry confirmed that anti-B7.1 mAb could bind to the protein encoded by this fragment, suggesting that the cDNA fragment is a splice isoform of B7.1 antigen.