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目的:分析霉菌性阴道炎和滴虫性阴道炎患者宫颈上皮细胞沙眼衣原体(CT)检出率判断出现混合感染的概率,以及阴道内混合感染对CT分离、纯化和药物敏感性的影响。方法:采用单层Hela-229细胞培养法和碘染色法对200名妇科门诊患者的宫颈上皮样本进行CT的分离、纯化和扩增,并检测其对阿奇霉素的药物敏感性。结果:霉菌性阴道炎混合感染CT者占18.3%,滴虫性阴道炎混合感染CT者占24.1%,较其他人群高(7.9%)。经反复传代后能获得高滴度CT临床株的,霉菌性阴道炎为66.7%,滴虫性阴道炎为71.4%,其他人群为14.3%。实验分离到的临床株未发现阿奇霉素耐药性,最小抑菌浓度范围为(0.078～0.625)μg/ml。结论:霉菌性阴道炎和滴虫性阴道炎患者的CT检出率高。经反复传代,混合感染分离的CT更易获得高滴度。混合感染有加重CT感染毒力的倾向,尚未出现阿奇霉素耐药株。
OBJECTIVE: To analyze the detection rate of chlamydia trachomatis (CT) in cervical epithelial cells from patients with fungal vaginitis and trichomonas vaginitis and to evaluate the effect of mixed infection on CT separation, purification and drug sensitivity. METHODS: Cervical epithelial samples of 200 gynecological outpatients were isolated, purified and amplified by single-layer Hela-229 cell culture and iodine staining. The drug sensitivity to azithromycin was tested. Results: The mixed infection of fungal vaginitis accounted for 18.3% of CT, trichomonas vaginitis mixed infection accounted for 24.1% of CT, higher than other groups (7.9%). After repeated passage to obtain high titer CT clinical strains, mycotic vaginitis was 66.7%, trichomonas vaginitis was 71.4%, the other population was 14.3%. Azithromycin resistance was not observed in the clinical isolates tested. The minimum inhibitory concentration ranged from 0.078 to 0.625 μg / ml. Conclusion: The detection rate of CT in patients with fungal vaginitis and trichomonas vaginitis is high. After repeated passages, mixed infected CT is more likely to obtain high titer. Mixed infection aggravate the tendency of CT infection virulence, azithromycin resistant strains have not yet emerged.