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目的 :研究丙型肝炎病毒E2基因接种能否诱导机体产生细胞免疫以及乙型肝炎病毒preS基因对其诱导细胞免疫能力的影响。方法 :以BALB/c小鼠的骨髓瘤细胞株 (SP2 /O)为宿主细胞 ,建立表达丙肝病毒E2蛋白的靶细胞 ,将其注射于E2基因或 preS与E2融合蛋白基因免疫的小鼠腹腔 ,记录小鼠接种瘤细胞后的存活期 ,以小鼠存活期的长短作为反映基因免疫小鼠对HCVE2蛋白细胞免疫的有无或强弱的指标。结果 :靶细胞表达了相对分子质量约为 70 0 0 0的E2蛋白 ,E2基因接种小鼠的存活期长于对照组 ,单独E2基因接种组的存活期显著长于对照组 ,也长于 preS与E2融合蛋白基因免疫组。 结论 :E2基因接种能诱导细胞免疫 ,但与 preS融合后 ,其诱导细胞免疫的能力会降低。
Objective: To investigate whether hepatitis C virus E2 inoculation can induce cell-mediated immunity and the influence of hepatitis B virus preS gene on the cellular immunity. Methods: BALB / c mouse myeloma cell lines (SP2 / O) were used as host cells to establish target cells expressing HCV E2 protein and then injected into the peritoneal cavity of mice immunized with E2 gene or preS and E2 fusion protein genes , Record the survival of mice after inoculation of tumor cells, the survival of mice as a reflection of the genetically immunized mice with HCVE2 protein cell immune index of the strength or weakness. Results: The target cells expressed E2 protein with a relative molecular mass of about 70 000. The survival of mice inoculated with E2 gene was longer than that of the control group. The survival of the mice inoculated with E2 gene alone was significantly longer than that in the control group, Protein gene immunization group. CONCLUSION: E2 vaccination can induce cellular immunity, but its ability to induce cellular immunity decreases after fusion with preS.